期刊
BIOSENSORS & BIOELECTRONICS
卷 55, 期 -, 页码 285-288出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.12.032
关键词
DNAzyme; Copper(II) ion; AuNRs; Fluorescence resonance energy transfer; technique; Fluorescent polarization
类别
资金
- National Natural Science Foundation of China [21165004, 21163002]
- Guangxi Natural Science Foundation of China [2010GXNSFF013001, 2012GXNSFBA0 53022, 2013GXNSFBA019044]
- project of Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University), Ministry of Education of China [CMEMR2011-14]
In the paper, we have constructed a very simple, sensitive and promising assay for fluorescence biosensor detection of Cu2+ in aqueous solutions based on FRET between gold nanorods (AuNRs) and the 3'-TAMRA-labeled substrate strand (Sub) of DNAzyme. The fluorescence of the Sub is quenched when the substrate strand-DNAzyme strand (Sub-Enz) duplex is adsorbed on AuNRs surface through electrostatic interaction. In the presence of Cu2+, the fluorescence is restored due to the decrease of FRET efficiency caused by the specific cleavage of the Sub by the DNAzyme (Enz), which weakens the electrostatic interaction between the AuNRs and short 3'-TAMRA-labeled DNA fragment. This method shows a high sensitivity for Cu2+ with a detection limit of 9.83 pM (SIN=3) and a linear range from 0.016 nM to 40 nM. At the same time, Cu2+ can be detected sensitively based on the significantly decreased Fluorescent polarization (FP). The detection limit of 8.40 pM is experimentally achieved for Cu2+. (C) 2013 Elsevier B.V. All rights reserved.
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