期刊
BIOSENSORS & BIOELECTRONICS
卷 59, 期 -, 页码 216-220出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.03.045
关键词
Dithiothreitol-capped gold nanoclusters; One-step facile synthesis; Copper(II) ion detection; Serum sample
类别
资金
- National Natural Science Foundation of China (NSFC) [21372097]
- Open Fund of the National Laboratory of Protein and Plant Gene Research at Peking University
- CIMO grant from Finland
We report here a Green method for the synthesis of fluorescent gold nanoclusters using dithiothreitol (DTT) as both a capping agent and reducing agent at 22 degrees C and pH 8. The physical and chemical properties of the synthesized AuNCs@DTT were studied by TEM and UV-vis absorption, fluorescence, and X-ray photoelectron spectroscopy. AuNCs@DTT recognizes cupric ions with high selectivity and sensitivity, which allows this material to act as a copper(II) sensor in aqueous solution. A linear relationship was observed between the fluorescence intensity of the DTT capped gold nanoclusters and the concentration of copper(II) ions, in the range of 0-60 mu M with a detection limit of 80 nM. The copper content in serum was also analyzed by using this copper sensor. It was shown that data obtained using the proposed method was comparable to values obtained by the traditional colorimetric method. This technique represents an alternative method for the determination of serum copper in clinical diagnosis especially for those laboratories which lack expensive analytical facilities. (C) 2014 Elsevier B.V. All rights reserved.
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