期刊
BIOSENSORS & BIOELECTRONICS
卷 61, 期 -, 页码 57-62出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.04.047
关键词
Protein biomarker; Electrochemical peptide sensor; Reagentless detection; XIAP-BIR3; Signal-off/signal-on
类别
资金
- China Scholarship Council (CSC)
- National Natural Science Foundation of China [J1210001]
- University Sorbonne Paris Cite (USPC) [ANR-11-IDEX-0005-02]
In this work, we report a reagentless electrochemical peptide (AVPFAQKG) sensor to directly detect the BIR3 domain of X-linked inhibitor of apoptosis protein (XIAP-BIR3). The bioreceptor was based on a conducting copolymer film electrosynthesized from juglone and a juglone-peptide conjugate UP) newly designed. The peptide-protein interactions generated an important increase of steric hindrance at the interface and a current decrease (signal off) of the redox reaction from quinone embedded in the polymer backbone as evidenced by Square Wave Voltammetry. This allowed a specific and sensitive detection of XIAP-BIR3 with a detection limit of 1 nM (13 ng mL(-1)). The peptide-protein complex could be then dissociated by adding the free precursor peptide (AVPFAQKG) into solution, causing a shift-back on the signal, i.e. an increase in the current intensity (signal-on). This off-on detection sequence was used in this work as a double verification of the specificity and this approach can be employed as a general way to increase the reliability of the results. In general, the approach described in this work may be inspired to develop other direct and reagentless electrochemical protein assays with high specificity and sensitivity. (C) 2014 Elsevier B.V. All rights reserved.
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