期刊
BIOSENSORS & BIOELECTRONICS
卷 62, 期 -, 页码 13-18出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.06.026
关键词
Thiocyanate detection; Serum; Saliva; Surface enhanced Raman scattering; Microfluidic
类别
资金
- National Science Foundation of China [60708024, 60877024, 61177033, 61275182]
- Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) [20070286058, 20090092110015]
- Scientific Research Foundation of Graduate School of Southeast University [YBJJ1328]
- Scientific Innovation Research Foundation of College Graduate in Jiangsu Province [CXZZ13_0088]
- Science Foundation for the Excellent Youth Scholars of Southeast University
- Fundamental Research Funds for the Central University
As thiocyanate (SCN-) acts as an important biomarker in human health assessment, there remains an urgent need to realize rapid and reproducible analysis of SCN- in body fluids. Here, a droplet microfluidic device has been designed and fabricated for SCN- detection in real human serum and saliva using the surface enhanced Raman scattering (SERS) technique. Only a few minutes are needed for the whole detection process which simply cost a few microliters of real sample. Gold@silver core-shell nanorods (Au@Ag NRs) with a large SERS enhancement factor were selected to capture SCN- ions in body fluids. The intensity of SERS peak at around 2100 cm(-1), which originates from the -C equivalent to N stretching mode, was used to indicate the concentrations of SCN- ions. Importantly, by generating a droplet environment for mixing reagents and acquiring signals, this microfluidic platform possesses the advantages of an improved reproducibility and reduced time consumption. For practical applications, the SERS-microfluidic system is capable to achieve rapid analysis of SCN- in the presence of human serum, which is very important for realizing the detection in real biological samples. Additionally, SCN- in saliva samples was detected in the SERS-microfluidic chip and the results provide useful information for distinguishing between smokers and nonsmokers. (C) 2014 Elsevier B.V. All rights reserved.
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