期刊
BIOSENSORS & BIOELECTRONICS
卷 61, 期 -, 页码 562-568出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.06.005
关键词
Cd-3[Co(CN)(6)](2) nanocubes; Cu-3[Co(CN)(6)](2) nanocubes; Electrochemical immunosensor; Carcinoembryonic antigen; Alpha-fetoprotein
类别
资金
- National Natural Science Foundation of China [21273153]
- Beijing Natural Science Foundation [2132008]
- Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges Under Beijing Municipality [IDHT20140512]
In this work, Cd-3[Co(CN)(6)](2) and Cu-3[Co(CN)(6)](2) (CdNCs and CuNCs) nanocubes were synthesized simply by a one-step process at room temperature in the presence of chitosan (CS). It was found that CdNCs and CuNCs produced obviously distinctive anodic peak currents at -0.7 V and -0.1 V (vs. Ag/AgCl), whose separation enabled differentiation between two analytes. They were used as novel electrochemical probes in multiplex electrochemical detection for carcinoembiyonic antigen (CEA) and alpha-fetoprotein (AFP) in a single run. The good performance of the new electrochemical probes was obtained. The linearity range was from 0.025 to 250 ng mL(-1) for both CEA and AFP. The detection limit of CEA was 0.0175 ng mL(-1) and that of AFP was 0.0109 ng mL(-1) at a signal-to-noise of 3. Analysis of clinical serum samples using this immunosensor was well consistent with the data determined by the enzyme-linked immunosorbent assay (ELISA). The novel electrochemical probes could be generally used in multiplex protein detections. (C) 2014 Elsevier B.V. All rights reserved.
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