期刊
BIOSENSORS & BIOELECTRONICS
卷 61, 期 -, 页码 547-553出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.06.001
关键词
AuNP; Au-C bonding; BoNT/A; Immunosensor; Signal amplification
类别
资金
- National Natural Science Foundation of China [209237057]
- CCNU from the colleges' basic research and operation of MOE [CCNU13A05006]
This work introduced an efficient approach for modification of AuNPs with multicomponents by diazonium salt couplings. The multifunctionalized AuNPs with protruding functional groups that allow simple bioconjugation to large amounts of biomolecules have been successfully used as electronic bridges and signal amplifiers for an electrochemical immunosensor towards the detection of BoNT/A. The one-step anchoring AuNPs strategy has greatly increased the efficiency for attachment of biomolecules and subsequently increased the sensitivity. Sensitivity was further amplified by preparation of bioconjugates particles containing horseradish peroxidase (HRP) labels along with detection antibodies (Ab(L)) attached to AuNPs. The immunosensor can be used for the detection of BoNT/A over the range of 4-35 pg mL(-1) with the lowest detection limit of 1 pg mL(-1) and assay time of 10 mm. The herein sensing strategy is rapid, robust, selective, sensitive, and is promising for future fabrication of point-of-care devices. (C) 2014 Elsevier B.V. All rights reserved.
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