期刊
BIOSENSORS & BIOELECTRONICS
卷 55, 期 -, 页码 209-215出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.12.007
关键词
Quantum dot; ZnO nanowire array; Immunoassay; Fluorescence resonance energy transfer; Carcinoembryonic antigen; Biosensing
类别
资金
- National Leading Research Laboratory Program [NRF-2013R1A2A1A05006378]
- Global Frontier Project [CISS-2012M3A6A6054201]
- National Research Foundation funded by the Ministry of Science, ICT and Future Planning of Korea [2012057022]
In this paper, we report an efficient and high-performance immunoassay platform by combining high-density vertical ZnO nanowire array with photostable quantum dot (QD) labeling. The ZnO nanowire array provides a large surface area for the immobilization of biomolecules, which makes it an efficient substrate for the immunoreaction of biomolecules. When a sandwich immunoassay with QD label was conducted on various substrates, the ZnO nanowire substrate showed stronger fluorescence signal than ZnO thin film and bare glass substrates by 3.8 and 8.5 times, respectively. We found that the fluorescence resonance energy transfer (FRET) from QD to ZnO nanowire could be suppressed by extending their distance with multilayer biotin-streptavidin complex. In addition, we demonstrated the QD-based immunoassay of carcinoembryonic antigen (CEA) on a ZnO nanowire substrate, showing an excellent immunoassay performance with a very low detection limit (0.001 ng/mL) and a large detection range up to 100 ng/mL. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据