期刊
BIOSENSORS & BIOELECTRONICS
卷 62, 期 -, 页码 331-336出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.07.005
关键词
BSA-PtNPs; Peroxidase-like activity; Protein antifouling property; Choline; Acetylcholine
类别
资金
- National Natural Science Foundation of China [21175023]
- Program for New Century Excellent Talents in University [NCET-12-0618]
- Natural Science Foundation of Fujian Province [2012J06019]
- Medical Elite Cultivation Program of Fujian [2013-ZQN-ZD-25]
Platinum nanoparticles (PtNPs) in the scaffold of bovine serum albumin (BSA) through biomineralization are found to possess excellent peroxidase-like activity that can catalyze N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt (TOPS) coupled with 4-amino-antipyrine (4-AAP) by the action of hydrogen peroxide to give an obvious purple product. Based on this phenomenon, acetylcholinesterase (AChE) and choline oxidase (ChOx) are used to catalyze ACh and choline to form the active product H2O2 and the asproduced H2O2 is detected optically. Owning to the protection effect of the protein shell, BSA-PtNPs turn out to be very stable and preserve the catalytic activity in the presence of protein and even in the real plasma samples. This protein antifouling property makes the BSA-PtNPs suitable for a wide range of applications in sensors for biological samples. Choline in infant formula and ACh in plasma have been successfully detected. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据