期刊
BIOSENSORS & BIOELECTRONICS
卷 51, 期 -, 页码 330-335出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.07.042
关键词
Mesoporous silica; Carbon dots; Fluorescent probe; Copper ions; L-cysteine; off-on detection
类别
资金
- National Natural Science Foundation of China [21236003, 21206042, 20925621, 20976054, 21176083]
- Fundamental Research Funds for the Central Universities
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0825]
- Shanghai Leading Academic Discipline Project [B502]
- China Scholarship Council
- Commonwealth Scientific and Industrial Research Organisation (CSIRO) Australia Function Interfaces Research Group
Copper ion (Cu2+) and L-cysteine (L-Cys) detection is critically important since an abnormal level of Cu2+ or L-Cys is an indicator for many diseases. In this paper, we demonstrate an off-on approach for highly sensitive and selective detection of Cu2+ and L-Cys using carbon dots (CDs) as fluorescent probes. CDs were prepared by using mesoporous silica (MS) spheres as nanoreactors. The binding ability of CDs towards metal ions was examined by comparing the fluorescence intensities of CDs before and after the addition of the metal ions. The addition of Cu2+ cations leads to their absorption on the surface of CDs and the significant fluorescence quench of CDs (turn-off). The resulting in CDs-Cu2+ system was found to be sensitive to L-Cys. The addition of L-Cys not only serves to shelter the CDs effectively from being quenched, but also to reverse the quenching and restore the fluorescence (turn-on) due to its ability to remove Cu2+ from the surface of CDs. This method is facile, rapid, low cost, and environment-friendly. A detection limit as low as 2.3 x 10(-8) M for Cu2+ and 3.4 x 10(-10) M for L-Cys is obtained, which is promising for biological applications. (C) 2013 Elsevier B.V. All rights reserved.
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