期刊
BIOSENSORS & BIOELECTRONICS
卷 61, 期 -, 页码 95-101出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.05.009
关键词
Fiber optics; Protein biomarker biosensors; Nanofabrication; Au nanodisk array; Localized surface plasmon resonance; Signal transduction
类别
资金
- National Science Foundation's Experimental Program to Stimulate Competitive Research (NSF EPSCoR) [34-21530-200-76190]
- Joint School of Nanoscience and Nanoengineering (JSNN)
A miniaturized, localized surface plasmon resonance (LSPR)-coupled fiber-optic (FO) nanoprobe is reported as a biosensor that is capable of label-free, sensitive detection of a cancer protein biomarker, free prostate specific antigen (f-PSA). The biosensor is based on the LSPR at the reusable dielectric-metallic hybrid interface with a robust, gold nano-disk array at the fiber end facet that is directly fabricated using EBL and metal lift-off process. The f-PSA has been detected with a mouse anti-human PSA monoclonal antibody (mAb) as a specific receptor linked with a self-assembled monolayer at the LSPR-FO facet surfaces. Experimental investigation and data analysis found near field refractive index (RI) sensitivity at similar to 226 nm/RIU with current LSPR-FO nanoprobe, and demonstrated the lowest limit of detection (LOD) at 100 fg/mL (similar to 3 fM) of f-PSA in PBS solutions. The control experimentation using 5 mg/mL bovine serum albumin in PBS and nonspecific surface test shows the excellent specificity and selectivity in the detection of f-PSA in PBS. These results present important progress towards a miniaturized, multifunctional fiber-optic technology that integrates informational communication and sensing function for developing a high performance, label-free, point-of-care (POC) device. (C) 2014 Elsevier B.V. All rights reserved.
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