期刊
BIOSENSORS & BIOELECTRONICS
卷 61, 期 -, 页码 9-13出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.04.036
关键词
Nanosensor; Quantum dots; Lysozyme; Fluorescence turn on-off
类别
资金
- National Natural Science Foundation of China, People's Republic of China [21075050, 21005029]
In this work, we developed an ultrasensitive turn on-off fluorescence nanosensor for lysozyme (Lyz) detection. The novel nanosensor was constructed with the carboxymethyl chitosan modified CdTe quantum dots (CMCS-QDs). Firstly, the CMCS-QDs were fabricated via the electrostatic interaction between amino groups in CMCS polymeric chains and carboxyl groups on the surface of QDs. In the fluorescence turn-on step, the strong binding ability between Zn2+ and CMCS on the surface of QDs can enhance the photoluminescence intensity (PL) of QDs. In the following fluorescence turn-off step, the N-acetyl-glucosamine (NAG) section along the CMCS chains was hydrolyzed by Lyz. As a result, Zn2+ was released from the surface of QDs, and the Lyz-QDs complexes were formed to quench the QDs PL. Under the optimal conditions, there was a good linear relationship between the PL of QDs and the Lyz concentration (0.1-1.2 ng/mL) with the detection limit of 0.031 ng/mL. The developed method was ultrasensitive, highly selective and fast. It has been successfully employed in the detection of Lyz in the serum with satisfactory results. (C) 2014 Elsevier B.V. All rights reserved.
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