期刊
BIOSENSORS & BIOELECTRONICS
卷 55, 期 -, 页码 51-56出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.11.056
关键词
Multi-analyte; Electrochemical immunoassay; Graphene nanoribbon; Polymer@metal nanocomposites; Heated screen-printed carbon electrode
类别
资金
- National Basic Research Program of China [2011CB933502]
- Anhui Provincial Natural Science Foundation [1208085MB27]
- research fund of Key Laboratory for Advanced Technology in Environmental Protection of Jiangsu Province [AE201107]
- Deanship of Scientific Research at King Saud University [RGP-VPP-029]
An ultrasensitive electrochemical immunoassay was developed for rapid detection of interleukin-6 (IL-6) and matrix metallopeptidase-9 (MMP-9); the method utilized PS@PDA-metal nanocomposites based on graphene nanoribbon (GNR)-modified heated screen-printed carbon electrode (HSPCE). Because of the good hydrophilicity and low toxicity, GNRs were used to immobilize antibodies (Ab) and amplify the electrochemical signal. PS@PDA-metal was used to label antibodies and generate a strong electrochemical signal in acetic buffer. A sandwich strategy was adopted to achieve simultaneous detection of MMP-9 and IL-6 based on HSPCE without cross-talk between adjacent electrodes in the range of 10(-5) to 10(3) ng mL(-1) with detection limits of 5 fg mL(-1) and 0.1 pg mL(-1) (S/N=3), respectively. The proposed method showed wide detection range, low detection limit, acceptable stability and good reproducibility. Satisfactory results were also obtained in the practical samples, thus showing this is a promising technique for simultaneous clinical detection of biocomponent proteins. (C) 2013 Elsevier B.V. All rights reserved.
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