期刊
BIOSENSORS & BIOELECTRONICS
卷 51, 期 -, 页码 97-102出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.07.025
关键词
Microfluidic chip; Flexible PDMS; Electrochemical cytosensing; Dynamic analysis; Apoptosis
类别
资金
- National Basic Research Program of China [2011CB933502]
- International S&T Cooperation Projects of China [2010DFA42060]
- National Natural Science Foundation of China [21121091, 21020102038]
A novel microfluidic platform integrated with a flexible PDMS-based electrochemical cytosensor was developed for real-time monitoring of the proliferation and apoptosis of HeLa cells. The PDMS-gold film, which had a conductive smooth surface and was semi-transparent, facilitated electrochemical measurements and optical microscope observations. We observed distinct increases and decreases in peak current intensity, corresponding to cell proliferation in culture medium and apoptosis in the presence of an anticancer drug, respectively. This electrochemical analysis method permitted real-time, label-free monitoring of cell behavior, and the electrochemical results were confirmed with optical microscopy. The flexible microfluidic electrochemical platform presented here is suitable for on-site monitoring of cell behavior in microenvironments. (C) 2013 Elsevier B.V. All rights reserved.
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