期刊
BIOSENSORS & BIOELECTRONICS
卷 54, 期 -, 页码 20-26出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.10.006
关键词
Electrochemiluminescence; Luminol; Hemin; Graphene; Nanoparticles; Glucose oxidase
类别
资金
- NNSF of China [21075100, 21275119]
- State Key Laboratory of Electroanalytical Chemistry [SKLEAC2010009]
- Ministry of Education of China [708073]
- Natural Science Foundation of Chongqing City [CSTC-2010BB4121, CSTC-2011BA7003]
- Specialized Research Fund for the Doctoral Program of Higher Education [20100182110015]
- Southwest China University [KB2010006]
A novel and ultrasensitive electrochemiluminescence (ECL) immunosensor, which was based on the amplifying ECL of luminol by hemin-reduced graphene oxide (hemin-rGO) and Ag nanoparticles (AgNPs) decorated reduced graphene oxide (Ag-rGO), was constructed for the detection of carcinoembryonic antigen (CEA). For this proposed sandwich-type ECL immunosensor, Au nanoparticles electrodeposited (DpAu) onto hemin-rGO (DpAu/hemin-rGO) constructed the base of the immunosensor. DpAu had outstanding electrical conductivity to promote the electron transfer at the electrode interface and had good biocompatibility to load large amounts of primary antibody (Ab(1)), which provided an excellent platform for this immunosensor. Moreover, AgNPs and glucose oxidase (GOD) functionalized graphene labeled secondary antibody (Ag-rGO-Ab(2)-GOD) was designed as the signal probe for the sandwiched immunosensor. Not only did the hemin-rGO improve the electron transfer of the electrode surface, but hemin also further amplified the ECL signal of luminol in the presence of hydrogen peroxide (H2O2). With the aid of Ag-rGO-Ab(2)-GOD, enhanced signal was obtained by in situ generation of H2O2 and catalysis of AgNPs to ECL reaction of the luminol-H2O2 system. The as-prepared ECL immunosensor exhibited excellent analytical property for the detection of CEA in the range from 0.1 pg mL(-1) to 160 ng mL(-1) with a detection limit of 0.03 pg mL(-1) (SN-1=3). (C) 2013 Elsevier B.V. All rights reserved.
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