期刊
BIOSENSORS & BIOELECTRONICS
卷 51, 期 -, 页码 191-194出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.07.053
关键词
Biosensor; DNA nanostructure; Impedance spectroscopy; Aptamer; Cocaine
类别
资金
- NSFC [21105080]
- SRFDP [20126101120023]
- NSF of Shaanxi Province [2012JQ2010, 2012JM2013, 2013KJXX-25]
- Scientific Research Foundation of Shaanxi Provincial Key Laboratory [11JS080, 12JS087]
- Scientific Research Program of Shaanxi Provincial Education Department [12JK0576]
We proposed an ultrasensitive electrochemical cocaine biosensor based on the three-dimensional (3D) DNA structure conversion of nanostructure from Triangular Pyramid Frustum (TPFDNA) to Equilateral Triangle (ETDNA). The presence of cocaine triggered the aptamer-composed DNA nanostructure change from Close to Open, leading to obvious faradaic impedance changes. The unique properties with excellent stability and specific rigid structure of the 3D DNA nanostructure made the biosensing functions stable, sensitive, and regenerable. The Faradaic impedance responses were linearly related to cocaine concentration between 1.0 nM and 2.0 mu M with a correlation coefficient of 0.993. The limit of detection was calculated to be 0.21 nM following IUPAC recommendations (3S(b)/b). It is expected that the distinctive features of DNA nanostructure would make it potentially advantageous for a broad range of biosensing, bionanoelectronics, and therapeutic applications. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据