期刊
BIOSENSORS & BIOELECTRONICS
卷 54, 期 -, 页码 585-597出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.10.075
关键词
Microfluidics; Point-of-care (POC); Paper-based diagnostics
类别
资金
- National 111 Project of China [B06024]
- Major International Joint Research Program of China [11120101002]
- Chinese Ministry of Education [313045]
- South Wisdom Valley Innovative Research Team Program
- International Science & Technology Cooperation Program of China [2013DFG02930]
- National Instrumentation Program [2013YQ190467]
- Fundamental Research Funds for the Central Universities
- China Postdoctoral Science Foundation [2013M532054]
- Science and Technology Research and Development Program
- Shaanxi Province of China [2012K08-18]
- Ministry of Higher Education (MOHE), Government of Malaysia [UM.C/HIR/MOHE/ENG/44]
- China Young 1000-Talent Program
- Program for New Century Excellent Talents in University [NCET-12-0437]
Advanced diagnostic technologies, such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), have been widely used in well-equipped laboratories. However, they are not affordable or accessible in resource-limited settings due to the lack of basic infrastructure and/or trained operators. Paper-based diagnostic technologies are affordable, user-friendly, rapid, robust, and scalable for manufacturing, thus holding great potential to deliver point-of-care (POC) diagnostics to resource-limited settings. In this review, we present the working principles and reaction mechanism of paper-based diagnostics, including dipstick assays, lateral flow assays (LFAs), and microfluidic paper-based analytical devices (mu PADs), as well as the selection of substrates and fabrication methods. Further, we report the advances in improving detection sensitivity, quantification readout, procedure simplification and multi-functionalization of paper-based diagnostics, and discuss the disadvantages of paper-based diagnostics. We envision that miniaturized and integrated paper-based diagnostic devices with the sample-in-answer-out capability will meet the diverse requirements for diagnosis and treatment monitoring at the POC. (C) 2013 Elsevier B.V. All rights reserved.
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