期刊
BIOSENSORS & BIOELECTRONICS
卷 53, 期 -, 页码 453-458出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.10.013
关键词
Nanopore sensing; Copper; Polyhistidine; Chelation; Biosensor
类别
资金
- Defense Advanced Research Projects Agency [HR0011-10-C-0226]
- National Institutes of Health [1R011HG005095]
- Department of Homeland Security [HSHQDC-09-C-00091]
We report a stochastic nanopore sensing method for the detection of Cu2+ ions. By employing a polyhistidine molecule as a chelating agent, and based on the different signatures of the events produced by the translocation of the chelating agent through an a-hemolysin pore in the absence and presence of target analytes, trace amounts of copper ions could be detected with a detection limit of 40 nM. Importantly, although Co2+, Ni2+, and Zn2+ also interacts with the polyhistidine molecule, since the event residence times and/or blockage amplitudes for these metal chelates are significantly different from those of copper chelates, these metal ions do not interfere with Cu2+ detection. This chelating reaction approach should find useful application in the development of nanopore sensors for other metal ions. (C) 2013 Elsevier B.V. All rights reserved.
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