期刊
BIOSENSORS & BIOELECTRONICS
卷 41, 期 -, 页码 871-874出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.08.060
关键词
Label-free detection; Luminescent probe; Lead(II) ion; Iridium(III) complex; G-quadruplex
类别
资金
- Hong Kong Baptist University [FRG2/11-12/009]
- Environment and Conservation Fund (ECF Project) [3/2010]
- Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU)
- Health and Medical Research Fund [HMRF/11101212]
- Research Grants Council [HKBU/201811, HKBU/204612]
- Science and Technology Development Fund, Macao SAR [001/2012/A]
- University of Macau [SRG013-ICMS12-LCH, MYRG091(Y1-L2)-ICMS12-LCH, MYRG121(Y1-L2)-ICMS12-LCH]
A label-free oligonucleotide-based luminescent switch-on assay has been developed for the selective detection of sub-nanomolar Pb2+ ions in aqueous solution and real water samples. An iridium(III) complex was employed as a G-quadruplex specific luminescent probe and a guanine rich DNA (PS2.M, 5'-GTG(3)TAG(3)CG(3)T(2)G(2)-3') was employed as recognition unit for Pb2+ ions. The PS2.M exists in a single-stranded conformation in the absence of Pb2+ ions, and the weak binding of the iridium(III) probe to ssDNA results in a weak luminescence signal. Upon binding to Pb2+ ions, the single-stranded DNA sequence (PS2.M) is induced into a G-quadruplex conformation, which greatly enhances the luminescence emission of the iridium(III) probe. The assay can detect Pb2+ ions in aqueous media with a limit of detection of 600 pM. It also exhibits good selectivity for Pb2+ ions over other heavy metal ions. Furthermore, the application of the assay for the detection of Pb2+ ions in spiked river water samples has been demonstrated. (C) 2012 Elsevier B.V. All rights reserved.
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