期刊
BIOSENSORS & BIOELECTRONICS
卷 46, 期 -, 页码 74-79出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.02.020
关键词
Functionalized C-60 nanoparticles; Supramolecular interaction; Dual signal amplification; Aptasensor
类别
资金
- NNSF of China [21105081, 21075100, 21275119]
- Research Fund for the Doctoral Program of Higher Education (RFDP) [20110182120010]
- Ministry of Education of China [708073]
- Specialized Research Fund for the Doctoral Program of Higher Education [20100182110015]
- Natural Science Foundation Project of Chongqing City [CSTC-2010BB4121, CSTC-2011BA7003, CSTC-2009BA1003]
- Fundamental Research Funds for the Central Universities, China [XDJK2010C062, XDJK2012A004]
This work reports a new supramolecular method for the synthesis of the amino and thiol groups functionalized C-60 nanoparticles (FC(60)NPs) with the large surface active sites and good water solubility. First, Prussian blue carried gold nanoparticles were decorated onto the surface of the obtained FC(60)NPs (abbreviated as Au@PB/FC60). Subsequently, the Au@PB/FC60 was labeled by detection aptamers and alkaline phosphatase to act as tracer. On the other hand, onion-like mesoporous graphene sheets and gold nanoparticles were utilized as the biosensor platform to immobilize a large amount of capture aptamers, owing to theirs porous structure and high surface-to-volume ratio. Based on the sandwich format, a dual signal amplification strategy based on multi-labeled functionalized C-60 nanohybrid as tracing tag has been successfully developed for platelet-derived growth factor B-chain electrochemical detection with a wide linear response in the range of 0.002-40 nM and a limit of detection of 0.6 pM (S/N=3). The proposed aptasensor demonstrated good specificity and high sensitivity, implying potential applications in bioanalysis and biomedicine. (C) 2013 Elsevier B.V. All rights reserved.
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