期刊
BIOSENSORS & BIOELECTRONICS
卷 43, 期 -, 页码 1-5出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.11.031
关键词
Cholesterol; Cupric oxide nanoparticles; Enzyme mimic; Chemiluminescence; Luminol
类别
资金
- National Natural Science Foundation of China [21175023]
- Natural Science Foundation of Fujian Province [2011J01034, 2012J06019]
- Science and Technology Planning Project of Fujian Province [2011Y0030]
- Program for New Century Excellent Talents in Fujian Province University [JA11102]
A chemiluminescent cholesterol sensor with good selectivity and enhanced sensitivity was constructed based upon the peroxidase-like activity of cupric oxide nanoparticles. Cupric oxide nanoparticles can catalyze the oxidation of luminol by H2O2, which was produced by the reaction of cholesterol and oxygen that was catalyzed by cholesterol oxidase. Therefore, the oxidation of cholesterol could be transduced into the chemiluminescence of luminol by combining these two reactions. Under the optimum conditions, the CL intensity was proportional to the concentration of cholesterol over the range of 0.625-12.5 mu M and a detection limit was 0.17 mu M. The applicability of proposed method has been validated by determination of cholesterol in milk powder and human serum samples with satisfactory results. (c) 2012 Elsevier B.V. All rights reserved.
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