期刊
BIOSENSORS & BIOELECTRONICS
卷 41, 期 -, 页码 322-327出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.08.047
关键词
C-reactive protein; CRP; Nanogap-embedded FET; FET-based biosensor; Point-of-care testing (POCT)
类别
资金
- National Research and Development Program (NRDP) [2011-0002182]
- Technology Innovation Program through the Korea Innovation Cluster Foundation
- Ministry of Knowledge Economy [A2010D-D013]
- Korea Ministry of Education, Science and Technology (MEST)
- Ministry of Knowledge Economy (MKE), Republic of Korea [A2010D-D013] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Most of point-of-care testing (POCT) to improve facilitates in diagnosis, treatment, and monitoring of patients. POCT technique has still remained a quantitatively and accurately detective effect. In this article, we demonstrated that real human C-reactive protein (CRP) in serum was detected for a chip-based point-of-care testing application based on a nanogap-embedded field effect transistor (FET), and the results were compared with those obtained via the enzyme-linked immunosorbent assay (ELISA) method. The limit of detection (LOD), determined from the standard curve, was 0.1 ng/ml, which is comparable to that of commercialized ELISAs. We evaluated that an improved detection range (0.1 ng/ml to 100 ng/ml) was achieved by comparing with commercialized ELISA. Control experiments to determine selectivity and to discern false-positive/false-negative rates were also performed. This report is the first description of the detection of CRP in human serum using a silicon-based biosensor. (C) 2012 Elsevier B.V. All rights reserved.
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