期刊
BIOSENSORS & BIOELECTRONICS
卷 48, 期 -, 页码 113-119出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.03.005
关键词
Molecularly imprinted polymers; Protein sensing; Fluorescent detection; Molecular recognition; Synthetic receptor
类别
资金
- Japan Society for the Promotion of Science (JSPS) [24651261]
- Japan Science and Technology Agency (JST) (Adaptable & Seamless Technology Transfer Program through Target-driven RD)
- Kobe University
- Grants-in-Aid for Scientific Research [24651261] Funding Source: KAKEN
Protein-imprinted polymers, capable of specific transduction of protein binding events into fluorescent signal change, were designed and synthesized by using dansyl ethylenediamine-conjugated O-acryloyl L-hydroxyproline (Hyp-En-Dans). Human serum albumin (HSA) was used as a model target protein and HSA-imprinted polymers (HSA-IP) were prepared on glass substrates. Specific fluorescence change was observed for HSA binding on the imprinted polymer thin film, whereas a weaker response was observed for other proteins, including bovine serum albumin, chymotrypsin, lysozyme, and avidin. The binding specificity was found to derive from the rigid structure of the hydrogen-bondable pyrrolidine moiety. Compared with SPR measurements, the non-specific binding caused by the polymer matrix and/or randomly located fluorescent monomer residues that did not compose specific binding sites did not contribute to the observed fluorescence change. These results revealed that the proposed protein-imprinting technique using Hyp-En-Dans could provide a highly selective protein-sensing platform, in which only specific binding events would be detected by fluorescent measurements. (C) 2013 Elsevier B.V. All rights reserved.
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