期刊
BIOSENSORS & BIOELECTRONICS
卷 49, 期 -, 页码 415-419出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.05.042
关键词
Prostate specific antigen (PSA); Au nanoparticle; Nanobiosensor; Enzymatic cleavage reaction; Protein detection
类别
资金
- Original Technology Research Program for Brain Science through the National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology
- Nuclear R&D program through the National Research Foundation
- Ministry of Education, Science and Technology, Korea
- Ministry of Land, Transport and Maritime Affairs, Korea
PSA (prostate-specific antigen) is one of the most widely used proteins for the diagnosis of breast and prostate cancer. Of note, PSA displays enzymatic activity for the specific peptide sequence HSSKLQ which it recognizes and cleaves. In this study, we developed a site-specific enzymatic-cleavage-reaction-based biosensor for the detection of PSA using fluorescein isothiocyanate (FITC)/peptide-conjugated gold (Au) nanoparticle complexes (FPANs). The FPANs do not initially fluoresce in the spectral region associated with the fluorophore, due to the quenching effect of the Au nanoparticles. When PSA was added to a solution containing the FPANs, PSA recognized and cleaved the specific sequence of the peptides attached to the Au nanoparticles. As a result, FITCs were separated from the Au nanoparticles and emitted strong fluorescence in their spectral region. Using this detection method, PSA was successfully detected as a function of concentration (10 pM-100 nM). This approach is superior to the immunoassay with respect to the performance of sensor, which is very rapid, simple, and one-step method for the detection of PSA and other protein markers can be measured for the early detection of several diseases. (C) 2013 The Author. Published by Elsevier B.V. All rights reserved.
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