期刊
BIOSENSORS & BIOELECTRONICS
卷 39, 期 1, 页码 226-230出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.07.046
关键词
AuPt alloy; Nanochain; Immunoassay; Sandwich-type; CEA
类别
资金
- National Natural Science Foundation of China [21173017, 50902007, 21275102]
- Natural Science Foundation of Jiangsu Province [BK2010341]
- 973 Program [2010CB934701]
The recent development in the nanotechnology has paved the way for a large number of alloyed nanomaterials and devices of desirable properties which have useful functions for electrochemical sensor and biosensor applications. In this paper, bimetallic AuPt nanochains were synthesized through a mild chemical method, with which anti-horseradish peroxidase-conjugated anti-carcinoembryonic antigen (HRP-anti-CEA-NCAuPt) was developed for electrochemical detection of carcinoembryonic antigen (CEA) in a sandwich-type immunoassay format. The alloyed nanocrystals exhibit not only sound signal amplification effect of Au nanoparticles, but also further new combination of interfacial, electrical and structural properties arising from the disparate AuPt components. As a result, the electrochemical signal was significantly amplified by using the HRP-anti-CEA-NCAuPt as tracer and hydrogen peroxide as enzyme substrate. The linear range of the developed immunosensor is 0.01-200 ng/mL and the detection limit is 0.11 pg/mL of CEA, which makes the biometallic nanochains promising candidates for the next-generation sandwich-type electrochemical immunoassays. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据