期刊
BIOSENSORS & BIOELECTRONICS
卷 47, 期 -, 页码 75-79出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.02.042
关键词
Molecular chaperone activity; HSP70; Cucurbituril; Electrochemical technique; Assay method
类别
资金
- National Science Fund for Distinguished Young Scholars [20925520]
- National Natural Science Foundation of China [81172503]
- Clinical Oncology Research Program of Medical Science and Technology Development Foundation in Health Department of Jiangsu Province [P200905]
In this work, we have proposed a novel method to specifically assay the molecular chaperone activity of HSP70 based on the HSP70-substrate peptide interaction. By selectively labeling the substrate peptide of HSP70 via host-guest interaction with two different cucurbituril species, the HSP70-substrate peptide interaction can be transduced into detectable signal readout. By using the signal readout, assay of the molecular chaperone activity of HSP70 can be achieved. Moreover, by using our method, chaperone activity of HSP70 can serve as a reliable indicator of drug resistance in cancer treatment The experimental results reveal that enhanced chaperone activity of HSP70 is observed in both drug-resistant cancer cell line and the serum of cancer victim subject to anti-cancer therapy. Therefore, the proposed method to assay the molecular chaperone activity of HSP70 can be a tool of efficiency in evaluating therapeutic response in HSP70-targeted cancer treatment. (C) 2013 Elsevier B.V. All rights reserved.
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