期刊
BIOSENSORS & BIOELECTRONICS
卷 45, 期 -, 页码 174-180出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.01.009
关键词
Biosensors; Immunosensor; Electrochemical impedance spectroscopy; Bacteria detection; Enterohemorrhagic E. coli O157:H7
类别
资金
- CIBER
- Instituto de Salud Carlos III
- European Regional Development Fund
- Commission for Universities and Research of the Department of Innovation, Universities, and Enterprise of the Generalitat de Catalunya [2009 SGR 505]
- CIBER-BBN [EUI2008-00172]
- Spanish Ministry of Science and Innovation (MICINN)
The presence of enterohemorrhagic Escherichia coli bacteria in food can cause serious foodborne disease outbreaks. Early detection and identification of these pathogens is extremely important for public health and safety. Here we present a highly sensitive label-free immunosensor for the detection of pathogenic E. coli O157:H7. Anti-E. coli antibodies were covalently immobilised onto gold electrodes via a self-assembled monolayer (SAM) of mercaptohexadecanoic acid and the pathogenic bacteria were detected by electrochemical impedance spectroscopy (EIS). Surface Plasmon Resonance (SPR) was used to monitor the antibody immobilisation protocol and antibody patterned surfaces were used to demonstrate the specificity of the antibody coated surfaces against the pathogenic bacteria. The immunosensor showed a very low limit of detection (2 CFU/mL) and a large linear range (3 x 10-3 X 10(4) CFU/mL). Finally, the selectivity of the sensor was demonstrated and no significant adsorption of Salmonella typhimurium was observed. (C) 2013 Elsevier B.V. All rights reserved.
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