期刊
BIOSENSORS & BIOELECTRONICS
卷 41, 期 -, 页码 12-29出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.09.025
关键词
Antibody; Conjugation; Hapten; Immunoassay; ELISA; Fluorescence; FRET; Immunosensor; Quantum dot
类别
资金
- Spanish Ministerio de Ciencia e Innovacion [AGL2009-12940-C02-01-02/ALI]
- FEDER
- Ministerio de Ciencia e Innovacion and the European Social Fund
Quantum dots (QDs) are semiconductor nanoparticles with very interesting optical properties, like high quantum yield or narrow and size-tuneable fluorescence spectra. Current applications of QDs are widespread, their use as fluorescence labels in bioassays being one of the most promising. These nanoparticles are usually conjugated to highly specific biomolecules like antibodies, oligonucleotides, enzymes or aptamers to improve assay selectivity. In this review, QD surface passivation, conjugation to biomolecules, and purification strategies are discussed with special emphasis to the development of QD-based immunoassays for the detection of low molecular weight compounds given the relevance of this sort of analytes in health, food safety, pharmaceutical, or environmental monitoring areas. The aim of this review is to summarise the main achievements attained so far and to initialise researchers in the field of antibody-based assays employing QDs as labels, such as fluorescence-linked immunosorbent assay (FLISA), fluorescence (or Forster) resonance energy transfer (FRET), immunochromatographic methods, and immunosensors. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据