期刊
BIOSENSORS & BIOELECTRONICS
卷 42, 期 -, 页码 545-549出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.11.024
关键词
OTA detection; Aptasensor; Real-time PCR; Food safety
类别
资金
- MOST [2012BAC01B07, 2012BAD29B05, 2012AA06A303, 2012BAD29B04, 2011BAK10B07, 2011BAK10B05, 2011BAK10B01, 2010AA06Z302, 2010DFB3047]
A simple and ultrasensitive method was developed for the detection of Ochratoxin A, utilizing an aptamer as a molecular recognition probe and real-time quantitative PCR (RT-qPCR) amplification of its complementary DNA as signal generators. Under the optimized conditions, the cycle threshold (Ct) increased linearly with 10-fold serial dilutions of Ochratoxin A (OTA) from 5 x 10(-6) to 5 ng mL(-1), with a limit of detection (LOD) of 1 fg mL(-1). The specificity of this aptasensor was considered to be excellent, as when tested against four other toxins it produced no obvious Ct value change. Furthermore, a satisfactory analyte concentration recovery was obtained from a series of concentrations of OTA spiked into red wine. Therefore, this highly sensitive approach shows a significant potential in a wide range of target analytes. (C) 2012 Elsevier B.V. All rights reserved.
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