期刊
BIOSENSORS & BIOELECTRONICS
卷 48, 期 -, 页码 238-243出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.04.008
关键词
Hairpin DNA; Aromatic amine; Electrochemical impedance spectroscopy
类别
资金
- Major State Basic Research Development Program [2009CB421605]
- National Natural Science Foundation of China [21073019, 20977009]
- National Science Foundation for Innovative Research Group [51121003]
- Fundamental Research Funds for the Central Universities
- Fostering Foundation of Beijing Normal University for the Excellent Ph.D. Dissertation [2009016]
The amino-substituted naphthalene compounds, such as 1,8-diaminonaphthalene (1,8-DANAP), 2,3-diaminonaphthalene (2,3-DANAP), 1,5-diaminonaphthalene (1,5-DANAP), 1-naphthylamine (1-NAP) and 2-naphthylamine (2-NAP), were investigated by electrochemical impedance spectroscopy (EIS), which was based on the interaction with hairpin DNA immobilized on the gold electrodes. Upon hairpin DNA interacting with the target chemicals, the charge transfer resistance (R-CT) of the hairpin DNA films was significantly decreased and the charge transfer resistance change (Delta R-CT) decreased in a sequence of Delta R-CT (1,8-DANAP)>Delta R-CT (2,3-DANAP)>Delta R-CT (1,5-DANAP)>Delta R-CT (1-NAP)>Delta R-CT (2-NAP). The Delta R-CT changes were due to the difference in the binding constant (K-SV) of the target chemicals to DNA. In addition, the interaction mechanism was further explored using 1,8-DANAP as a model analyte by fluorescence spectra, Raman spectroscopy, differential pulse voltammetry (DPV) and EIS, correspondingly. The results demonstrated that the amino-substituted naphthalene compounds intercalated into stem appearing in the hairpin DNA. Moreover, the hairpin DNA sensor exhibited high sensitivity to the amino-substituted naphthalene compounds with the detection limit of nano-mole, and maintained high selectivity over other selected environmental pollutants. Finally, the DNA sensor was challenged in natural water sample with a recovery of 96-102%, which offered a platform for prospective future development of a simple, rapid, sensitive and low-cost assay for the detection of target aromatic amine pollutants. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.
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