期刊
BIOSENSORS & BIOELECTRONICS
卷 39, 期 1, 页码 112-117出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.06.056
关键词
Magnetic relaxation switch (MRS); Superparamagnetic iron oxide (SPIO) nanoparticles; Biotin-streptavidin system (B-SA); Kanamycin (KM); Immunosensor
类别
资金
- Commonwealth Scientific Foundation for Industry of the Chinese Inspection and Quarantine [201010022]
- 11th Five Years Key Programs for Science and Technology Development of China [2009BAK61B04]
A rapid, sensitive, and simple immunosensor was developed for the detection of Kanamycin (KM) in milk. This immunosensor is based on magnetic relaxation switch (MRS) assay and biotin-streptavidin system (B-SA system). The target analyte (KM) competed with those on the surface of the superparamagnetic iron oxide (SPIO) nanoparticles and hence affected the formation of SPIO aggregates. The dispersed and aggregated states of SPIO can modulate the spin-spin relaxation time (T-2) of the neighboring water molecule. T-2 was then changed as an effect of the target analyte. The B-SA system was used to amplify the SPIO binding, thus enhance the sensitivity. The detection working was 1.5 to 25.2 ng mL(-1) and limit of detection (LOD) was determined to be 0.1 ng mL(-1). The LOD of the immunosensor decreased tenfold, and its analysis time (45 min) was much shorter than that of enzyme-linked immunosorbent assay (6 h to 8 h). The average recoveries of the KM at various spiking levels ranged from 80.2% to 85.6% with a relative standard deviation (RSD) below 4.0%. The results showed that the MRS immunosensor was a promising platform for the determination of small molecular residues because of its high sensitivity, specificity, homogeneity, and speed. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.
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