期刊
BIOSENSORS & BIOELECTRONICS
卷 49, 期 -, 页码 466-471出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.06.009
关键词
Graphene oxide; Surface functionalization; Polydopamine; NADH; Alcohol dehydrogenase; AuNPs
类别
资金
- National Nature Sciences Foundation of China [21175114]
- Natural Science Foundation of Jiangsu [BK2011441]
- Qing Lan Project of Jiangsu Province
- NJUST Research Funding [2011ZDJH17, 2012ZDJH009]
- Fundamental Research Funds for the Central Universities [30920130112012]
We report here an efficient approach to enhance the performance of biosensing platform based on graphene or graphene derivate. Initially, graphene oxides (GO) nanosheets were reduced and surface functionalized by one-step oxidative polymerization of dopamine in basic solution at environment friendly condition to obtain the polydopamine (Pdop) modified reduced graphene oxides (PDRGO). The bioinspired surface was further used as a support to anchor active gold nanoparticles (AuNPs). The morphology and structure of the as-prepared AuNPs/PDRGO nanocomposite were investigated by field-emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), Fourier transform-infrared spectroscopy (FT-IR). Electrochemical studies demonstrate that the as-prepared AuNPs/PDRGO hybrid materials possess excellent electrochemical properties and electrocatalytic activity toward the oxidation of NADH at low potential (0.1 V vs. SCE) with the fast response (15 s) and the broad linear range (5.0 x 10(-8)-4.2 x 10(-5) M). Thus, this AuNPs/PDRGO nanocomposite can be further used to fabricate a sensitive alcohol biosensor using alcohol dehydrogenase (ADH), by simply incorporating the specific enzyme within the composite matrix with the aid of chitosan (Chit). (C) 2013 Elsevier B.V. All rights reserved.
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