期刊
BIOSENSORS & BIOELECTRONICS
卷 43, 期 -, 页码 205-210出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.12.016
关键词
Gold nanoparticles; Single-walled carbon nanotubes; Peroxidase mimetic; Biosensing
类别
资金
- National Natural Science Foundation of China [21075080, 21275096]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1070]
We prepared a new positively-charged gold nanoparticle ((+)AuNPs)-single-walled carbon nanotubes (SWNTs) nanohybrids. The results showed that small (+)AuNPs dispersed uniformly on the surface of SWNTs. Importantly the resulting nanohybrids exhibited fascinating peroxidase-like activity, which can catalyze oxidation of the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) by H2O2 to develop a blue color in aqueous solution. Furthermore, single-stranded DNA (ss-DNA) can resist salt-induced (+)AuNPs-SWNTs nanohybrids aggregation, whereas double-stranded DNA (ds-DNA) can not inhibit salt-induced nanohybrids aggregation. Based on these unique properties of the (+)AuNPs-SWNTs nanohybrids, we developed a label-free colorimetric method for DNA hybridization detection. The response to target DNA concentration was linear in the range of 0.025-0.5 mu M with a detection limit of 2 nM (3 sigma). Based on the specific recognition of aptamer, the method can be extended to detect non-nucleic acid targets such as cocaine. The present limit of detection for cocaine is 2 nM. The method offers the advantages of simple, cheap, rapid and sensitive. (c) 2012 Elsevier B.V. All rights reserved.
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