期刊
BIOSENSORS & BIOELECTRONICS
卷 42, 期 -, 页码 225-228出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.10.070
关键词
DNAzymes; Cu2+; Fluorescent sensor; Temperature variation tolerance; Ionic strength variation tolerance
类别
资金
- National Basic Research Program of China [2011CB707703]
- Natural Science Foundation of China [21175072]
- Fundamental Research Funds for the Central Universities
- Program for New Century Excellent Talents in University [NCET-10-0504]
- Chinese Ministry of Education
- Tianjin Normal University [52X09006]
A previously reported Cu2+-dependent DNAzyme/substrate complex was reconstructed in this work, which makes possible the use of an intramolecular stem-loop structure and is, therefore, a good choice for the design of Cu2+ sensors. To demonstrate this, a fluorescent sensor was designed on the basis of the reconstructed complex. In this sensor, the fluorophore/quencher pair was caged tightly in an intramolecular double-helix structure; thus, the background signal was greatly suppressed. Cu2+-dependent cleavage of the complex could cause the release of the fluorophore, leading to restoration of the fluorescence signal. High quenching efficiency provides the sensor with three important characteristics: high sensitivity, high temperature variation tolerance and high ionic strength tolerance. The proposed sensor allows specific detection of aqueous Cu2+ down to a limit of 0.6 nM, and the performance is independent of temperature and ionic strength in the range of 4-40 degrees C and 0.8-3.0 M NaCl, respectively. This work identifies a good choice for sensor design on the basis of DNAzymes containing triple-helix structures. (C) 2012 Elsevier B.V. All rights reserved.
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