4.8 Article

Hydrogen peroxide detection at a horseradish peroxidase biosensor with a Au nanoparticle-dotted titanate nanotube|hydrophobic ionic liquid scaffold

期刊

BIOSENSORS & BIOELECTRONICS
卷 32, 期 1, 页码 188-194

出版社

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.12.002

关键词

Gold nanoparticle-dotted TiO2 nanotubes; TiO2-ionic liquid composite materials; Electron transfer of HRP; Detection of H2O2

资金

  1. Henan Science and Technology Department [084300510023, 092102310282]

向作者/读者索取更多资源

In this work, a novel sensing scaffold, consisting Au nanoparticle (GNP)-dotted TiO2 nanotubes (TNTs) as the rigid material and the hydrophobic ionic liquid (HIL), 1-decyl-3-methylimidazolium tetrafluoroborate, as the entrapping agent, was applied to facilitate the electron transfer of horseradish peroxidase (HRP) on a glassy carbon electrode. GNPs were immobilised on the TNTs in our work using a one-step reduction of HAuCl4 center dot 3H(2)O by sodium borohydride in the presence of sodium citrate as a stabilising reagent. The morphology and composition of the as-synthesised composite materials were characterised by transmission electron microscopy, scanning electron microscopy, X-ray diffraction and Fourier-transform infrared spectroscopy. Cyclic voltammetry of HRP at the modified electrode presented a pair of reproducible, quasi-reversible redox peaks with a peak-to-peak separation of 69 mV, indicating electron transfer between HRP and composite electrode. The GNP-TNT vertical bar HIL vertical bar HRP electrode was then applied to the detection of H2O2 in a pH 7.0 phosphate buffer using chronoamperometry. The biosensor exhibited a linear response in the 15-750 mu M range, and a limit of detection of 2.2 mu M. The biosensor also exhibited stability with 90% of the detection signal retained over a two-week duration. (C) 2011 Elsevier B.V. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据