期刊
BIOSENSORS & BIOELECTRONICS
卷 36, 期 1, 页码 29-34出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.03.034
关键词
DNA aptamer sensor; Kanamycin; Label-free; Self-assemble; Conducting polymer; Gold nanoparticle
类别
资金
- Researcher Program through National Research Fund Grant
- Ministry of Education, Science and Technology, South Korea [20100029128]
- National Research Foundation of Korea [2009-0079376] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Highly sensitive label-free detection of kanamycin is achieved with an aptamer sensor based on a conducting polymer/gold self-assembled nanocomposite. The sensor probe is fabricated by covalently immobilizing an in vitro selected DNA aptamer for kanamycin onto gold nanoparticle (AuNP)-comprised conducting polymer, poly-[2, 5-di-(2-thienyl)-1H-pyrrole-1-(p-benzoic acid)] (poly-DPB). The self-assembling of DPB on AuNP is investigated by TEM and UV-vis spectroscopy and the modification of the aptamer sensor is characterized using XPS and electrochemical impedance spectroscopy. The probe is applied to detect kanamycin by using voltammetric techniques. The sensor shows a pair of redox peaks around 0.26/ 0.08 V (vs. Ag/AgCl) for kanamycin captured by the aptamer-immobilized probe. The parameters that can affect the response, such as aptamer concentration, incubation time, temperature, and pH are optimized. The calibration plot shows a linear range from 0.05 mu M to 9.0 mu M kanamycin with a detection limit of 9.4 +/- 0.4 nM. The proposed aptamer sensor is examined with a real sample. (C) 2012 Elsevier B.V. All rights reserved.
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