期刊
BIOSENSORS & BIOELECTRONICS
卷 32, 期 1, 页码 208-212出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.12.011
关键词
Ochratoxin A; Aptamer; G-quadruplex; DNAzyme; Hairpin
类别
资金
- National Natural Science Foundation of China [20890022]
- National Key Basic Research Development Project of China [2010CB933602]
- Scientific Office of the French Embassy in China
We report an aptasensor for biosensing of Ochratoxin A (OTA) using aptamer-DNAzyme hairpin as biorecognition element. The structure of this engineered nucleic acid includes the horseradish peroxidase (HRP)-mimicking DNAzyme and the OTA specific aptamer sequences. A blocking tail captures a part of these sequences in the stem region of the hairpin. In the presence of OTA, the hairpin is opened due to the formation of the aptamer-analyte complex. As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10 nM, showing a limit of detection of 2.5 nM. (C) 2011 Elsevier B.V. All rights reserved.
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