期刊
BIOSENSORS & BIOELECTRONICS
卷 34, 期 1, 页码 112-117出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.01.026
关键词
Clenbuterol; AuNPs; Melamine; Hydrogen-bonding
类别
资金
- Major National Scientific Research Plan of China (973 Program) [2011CB933202]
- Key Projects in the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period [2009BAK61B01]
- National Sci-Tech Major Special Item for Water Pollution Control and Management [2009ZX07527-007-003]
- State Key Laboratory of Electroanalytical Chemistry [SKLEAC2010005]
- State Key Laboratory of Environmental Chemistry and Ecotoxicology [KF2010-23]
- National Natural Science Foundation of China [20877099, 20972183]
A highly sensitive method for the detection of trace amount of clenbuterol based on gold nanoparticles (AuNPs) in the presence of melamine was described in this paper. Hydrogen-bonding interaction between clenbuterol and melamine resulted in the aggregation of AuNPs and a consequent color change of AuNPs from wine red to blue. The concentration of clenbuterol could be determined with naked eye or a UV-vis spectrometer. Results showed that the absorption ratio (A(670)/A(520)) was liner with the logarithm of clenbuterol concentration in the range of 2.8 x 10(-10) to 2.8 x 10(-7) M and 2.8 x 10(-7) to 1.4 x 10(-6) M with linear coefficients of 0.996 and 0.993, respectively. The detection limit was 2.8 x 10(-11) M (S/N = 3), which was much lower than most existing methods. The coexisting substances including in-epinephrine, phenylalamine, tryptohan, alamine, uric acid, glycine, glycerol, glucose, MgCl2, CaCl2 and NaCl did not affect the determination of clenbuterol. The proposed method could be successfully applied to the determination of clenbuterol in human urine. (C) 2012 Elsevier B.V. All rights reserved.
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