期刊
BIOSENSORS & BIOELECTRONICS
卷 33, 期 1, 页码 228-232出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.01.006
关键词
DNA biosensor; Electrochemical biosensing; Sequence analysis of DNA; Electrochemically reduced graphene; Signal amplification; Carbon sphere
类别
资金
- National Basic Research Program of China [2010CB732400]
- National Natural Science Foundation of China [20875044, 21075055, 21135002]
- Department of Health of Jiangsu Province
An ultrasensitive electrochemical DNA biosensor was constructed by assembling probe labeled gold nanoparticles (ssDNA-AuNP) on electrochemically reduced graphene oxide (ERGO) modified electrode with thiol group tagged (GT) DNA strand (d(GT)(29)SH) and coupling with horseradish peroxidase (HRP) functionalized carbon sphere (CNS) as tracer. The heteronanostructure formed on the biosensor surface appeared relatively good conductor for accelerating the electron transfer, while the HRP tagged CNS provided dual signal amplification for electrochemical biosensing. The triplex signal amplification strategy produced an ultrasensitive electrochemical detection of DNA down to attomolar level (5 aM) with a linear range of 5 orders of magnitude (from 1 x 10(-17) M to 1 x 10(-13) M), and appeared high selectivity to differentiate single-base mismatched and three-base mismatched sequences of DNA. The proposed approach provided a simple and reliable method for DNA detection with high sensitivity and specificity, indicating promising application in bioanalysis and biomedicine. (C) 2012 Elsevier B.V. All rights reserved.
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