期刊
BIOSENSORS & BIOELECTRONICS
卷 34, 期 1, 页码 286-290出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.11.035
关键词
Human serum albumin; Quantum dot; Optical immunosensor; Photodiode; 3-Aminopropyltriethoxysilane; Diode laser
类别
资金
- NSC [NSC98-2120-M-007-003]
In this study, a CdSe/ZnS quantum dot (QD)-based immunosensor using a simple optical system for human serum albumin (HSA) detection is developed. Monoclonal anti-HSA (AHSA) immobilized on 3-aminopropyltriethoxysilane (APTES)-modified glass was used to capture HSA specifically. Bovine serum albumin (BSA) was used to block non-specific sites. The solution, containing AHSA-QD complex prepared by mixing biotinylated polyclonal anti-HSA and streptavidin coated QD, was used to conjugate with the HSA molecules captured on AHSA/BSA/APTES-modified glass for the modification of HSA with QD. A simple optical system, comprising a diode laser (405 nm), an optical lens, a 515-nm-long pass filter, and an Si-photodiode, was used to detect fluorescence and convert it to photocurrent. The current intensity was determined by the amount of QD specifically conjugated with HSA, and was therefore HSA-concentration-dependent and could be used to quantify HSA concentration. The detection limit of the pure QD solution was similar to 3.5 x 10(-12) M, and the detection limit for the CdSe/ZnS QD-based immunosensor developed in this study was approximately 3.2 x 10(-5) mg/ml. This small optical biosensing system shows considerable potential for future applications of on-chip liver-function detection. (c) 2011 Elsevier B.V All rights reserved.
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