期刊
BIOSENSORS & BIOELECTRONICS
卷 35, 期 1, 页码 407-412出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.03.029
关键词
G-quadruplex DNAzyme; Antioxidant capacity; ATP; ABTS; Free radical
类别
资金
- National Basic Research Program of China [2011CB707703]
- Natural Science Foundation of China [21175072, 20975055]
- Chinese Ministry of Education [NCET-10-0504]
The scavenging of 2,2'-azinobis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radical cation (ABTS') by antioxidants has been widely used in antioxidant capacity assay. Because of ABTS(center dot+) disproportionation, however, this radical cannot be prepared on a large scale and stored long-term, making it unsuitable for high-throughput detection and screening of antioxidants. We developed a modified post-additional antioxidant capacity assay. This method possessed two remarkable features: First, instead of natural peroxidases, an artificial enzyme, G-quadruplex DNAzyme, was used for the preparation of ABTS(center dot+), thus greatly reducing the cost of the assay, and eliminating the strict demand for the storage of enzymes. Second, an ABTS(center dot+) stabilizer, adenosine triphosphate (ATP), was used. In the presence of ATP, the disproportionation of ABTS(center dot+) was effectively inhibited, and the lifetime of this radical cation was prolonged about 6-fold (12 days versus 2 days), making the large-scale preparation of ABTS(center dot+) possible. Utilizing this method, the antioxidant capacities of individual antioxidants and real samples can be quantified and compared easily. In addition, this method can be developed as a high-throughput screening method for antioxidants. The screening results could even be judged by the naked eye, eliminating the need for expensive instruments. (C) 2012 Elsevier B.V. All rights reserved.
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