期刊
BIOSENSORS & BIOELECTRONICS
卷 33, 期 1, 页码 29-35出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.11.054
关键词
Carbon nanotubes; Mesoporous silica nanoparticles; Grapheme; Electrochemical immunosensor; Human chorionic gonadotrophin
类别
资金
- Natural Science Research Foundation of China [21175058]
- Natural Science Foundation of Shandong Province, China [ZR2011BQ019]
- Technology Development Plan of Shandong Province, China [2011GGB01153]
A facile and sensitive electrochemical immunosensor for detection of human chorionic gonadotrophin (hCG) was designed by using functionalized mesoporous nanoparticles as bionanolabels. To construct high-performance electrochemical immunosensor, Au nanoparticles (AuNPs) dotted carbon nanotubes (MWCNTs)-graphene composite was immobilized on the working electrode, which can increase the surface area to capture a large amount of primary antibodies (Ab(1)) as well as improve the electronic transmission rate. The as-prepared bionanolabels. composed of mesoporous silica nanoparticles (MCM-41) coated with AuNPs through thionine linking, showed good adsorption of horseradish peroxidase-labeled secondary anti-hCG antibody. Interlayer thionine was not only a bridging agent between MCM-41 and AuNPs but also an excellent electron mediator. The approach provided a good linear response range from 0.005 to 500 mIU mL(-1) with a low detection limit of 0.0026 mIU mL(-1). The immunosensor showed good precision, acceptable stability and reproducibility. Satisfactory results were obtained for determination of hCG in human serum samples. The proposed method provides a new promising platform of clinical immunoassay for other biomolecules. (C) 2011 Elsevier B.V. All rights reserved.
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