期刊
BIOSENSORS & BIOELECTRONICS
卷 35, 期 1, 页码 251-257出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.02.057
关键词
Electrochemical immunosensor; Signal amplification; Graphene oxide nanosheet; Quantum dots; Tumor biomarkers
类别
资金
- UQ [RM 2010001241]
- UQ ECR [RM 2010002245]
- National Breast Cancer Foundation
A sensitive electrochemical immunosensor for the detection of epithelial cell adhesion molecule (EpCAM) antigen, a common marker for tumors of epithelial origin, employing bionanoconjugates as signal-transduction labels has been developed. The bionanoconjugates were fabricated by carboxylation of the two-dimensional graphene oxide nanosheets (GRs) and immobilizing streptavidin and amine-functionalized CdSe quantum dots (QDs) on carboxylated GRs via carbodiimide coupling chemistry, followed by the immunoreaction with the biotinylated secondary antibodies. Since carboxylated GRs have a higher density of active sites, it allows a large number of CdSe QDs to be immobilized onto the surface of the bionanoconjugates, and hence, enhance the sensitivity the immunosensor. The method enabled detection limits of 100 fg/mL and 1 pg/mL (based on the S/N = 3) in PBS buffer and serum samples, respectively, using anodic stripping voltammetric readout. The immunosensor showed a good selectivity, reproducibility, and long-storage stability, and may become a promising technique for the early detection of tumor biomarker in clinical/biological samples. (C) 2012 Elsevier B.V. All rights reserved.
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