期刊
BIOSENSORS & BIOELECTRONICS
卷 35, 期 1, 页码 82-86出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.02.015
关键词
Adrenocorticotropin hormone; Phenyl boronic acid; Electrochemical immunosensor
类别
资金
- Spanish Ministry of Science and Innovation
- Spanish Ministerio de Ciencia e Innovacion [DPS2008-07005-C02-01, CTQ2009-12650]
- Comunidad de Madrid [S2009/PPQ-1642]
This work reports for the first time an electrochemical immunosensor for the determination of adrenocorticotropin hormone (ACTH). The immunoelectrode design involves the use of amino phenylboronic acid for the oriented immobilization of anti-ACTH antibodies onto screen-printed carbon modified electrode surfaces. A competitive immunoassay between the antigen and the biotinylated hormone for the binding sites of the immobilized antibody was performed. The electroanalytical response was generated by using alkaline phosphatase-labelled streptavidin and 1-naphtyl phosphate as the enzyme substrate. The electrochemical oxidation of the enzyme reaction product, 1-naphtol, measured by differential pulse voltammetry was employed to monitor the affinity reaction. Under the optimized working conditions, an extremely low detection limit of 18 pg/L was obtained. Cross-reactivity was evaluated against other hormones (cortisol, estradiol, testosterone, progesterone, hGH and prolactin) and the obtained results demonstrated an excellent selectivity. The developed immunosensor was applied to a human serum sample containing a certified amount of ACTH with good results. (c) 2012 Elsevier B.V. All rights reserved.
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