期刊
BIOSENSORS & BIOELECTRONICS
卷 33, 期 1, 页码 69-74出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.12.033
关键词
Enzyme-linked immunosorbent assay; Manganese Oxide; Bacteria detection; Nanomaterials; Enzyme mimetics
类别
资金
- National Natural Science Foundation of China [41076047]
- Chinese Academy of Sciences [KZCX2-YW-205-03]
- Shandong Provincial Natural Science Foundation, China [ZR2010DM004]
Nanomaterial-based enzyme-linked immunosorbent assay (ELISA) with sufficient sensing specificity is a useful analytical tool for the detection of toxicologically important substances in complicated biological systems. Increasing worldwide demand for nanomaterials and increasing concern on their safe development and use, require a simple, stable, and sensitive detection assay for pathogen evaluation and environmental monitoring. However, this goal is not yet achieved. A design for a hybrid MnO2 nanowire-ELISA using the sandwich assay format, which provides quantitative binding information for both a specific antibody and the pathogen, sulfate-reducing bacteria, and detects pathogen concentration, is presented. 3,3',5,5'-Tetramethylbenzidine was used as the substrate and was allowed to react with the MnO2 nanowires without H2O2 in the reaction system. The kinetic parameters were measured with the system acting as a catalytic biosensor. The effectiveness of the MnO2 nanowire-based biosensor was demonstrated by its sensitive detection of the pathogen. (C) 2011 Elsevier B.V. All rights reserved.
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