期刊
BIOSENSORS & BIOELECTRONICS
卷 31, 期 1, 页码 151-156出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.10.008
关键词
Pd nanoparticle assemblies; Substitute of HRP; Biosensor; H2O2; Glucose
类别
资金
- National Natural Science Foundation of China [20875046, 21175069, 20871070, 20901041]
- Program for New Century Excellent Talents in University [NCET-09-0159]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
The spherical porous Pd nanoparticle assemblies (NPAs) have been successfully synthesized by starch-assisted chemical reduction of Pd(II) species at room temperature. Such Pd NPAs are not simply used to enlarge the surface area and to promote the electron transfer. They also catalyze the reduction of H2O2 which are regarded as horseradish peroxidase (HRP) substitutes in electron transfer process. By using them as electrocatalysts, as low as 6.8 x 10(-7) M H2O2 can be detected with a linear range from 1.0 x 10(-6) to 8.2 x 10(-4) M. Moreover, through co-immobilization of such Pd NPAs and glucose oxidase (GOx), a sensitive and selective glucose biosensor is developed. The detection principle lies on measuring the increase of cathodic current by co-reduction of dissolved oxygen and the in situ generated H2O2 during the enzymatic reaction. Under optimal conditions, the detection limit is down to 6.1 x 10(-6) M with a very wide linear range from 4.0 x 10(-5) to 2.2 x 10(-2) M. The proposed biosensor shows a fast response, good stability, high selectivity and reproducibility of serum glucose level. It provides a promising strategy to construct fast, sensitive, stable and anti-interferential amperometric biosensors for early diagnosis and prevention of diabetes. (C) 2011 Elsevier B.V. All rights reserved.
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