期刊
BIOSENSORS & BIOELECTRONICS
卷 32, 期 1, 页码 163-168出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.11.048
关键词
Self-assembled monolayers; DNA biosensor; Label-free; Reagentless; Signal-on; PCR product; Mycobacterium tuberculosis
类别
资金
- French Ministry of Research
- Vietnamese Ministry of Education and Training
- University of Paris-Sud 11
- French General Delegation for Armaments (DGA)
We report a signal-on, label-free and reagentless electrochemical DNA biosensor, based on a mixed self-assembled monolayer of thiolated hydroxynaphthoquinone and thiolated oligonucleotide. Electrochemical changes resulting from hybridization were evidenced with oligonucleotide targets (as models), as well as with polymerase chain reaction (PCR) products related to different lineages of Mycobacterium tuberculosis strains. With pure oligonucleotides, this system achieves high sensitivity (similar to 300 pM of DNA target, i.e. 30 fmol in a 100 mu l sample) and excellent selectivity, allowing to detect a single mismatch on a sequence of 20 bases. With PCR products, current changes are specific to the bacterial strain from which the PCR fragment is produced. In addition, the sensor response is of the signal-on type, giving a positive signal change upon hybridization, and therefore does not suffer from false positive responses due to non-specific adsorption of DNA. (C) 2011 Elsevier B.V. All rights reserved.
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