期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 11, 页码 4375-4381出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.04.044
关键词
Microfluidic; Electrochemical; IDA; PAP; beta-Galactosidase; DNA hybridization
类别
资金
- NIAID/NIH [2 U54 A1057168-06]
- United States Army [W81XWH0920109]
- NIBIB [5K25EB006011]
- Middle Atlantic RCE Program
A novel enzyme-linked DNA hybridization assay on an interdigitated array (IDA) microelectrode integrated into a microfluidic channel is demonstrated with sub-nM detection limit. To improve the detection limit as compared to conventional electrochemical biosensors, a recyclable redox product, 4-aminophenol (PAP) is used with an IDA microelectrode. The IDA has a modest and easily fabricated inter-digit spacing of 10 mu m, yet we were able to demonstrate 97% recycling efficiency of PAP due to the integration in a microfluidic channel. With a 70 nL sample volume, the characterized detection limit for PAP of 1.0 x 10(-10) M is achieved, with a linear dynamic range that extends from 1.0 x 10(-9) to 1.0 x 10(-5) M. This detection limit, which is the lowest reported detection limit for PAP, is due to the increased sensitivity provided by the sample confinement in the microfluidic channel, as well as the increased repeatability due to perfectly static flow in the microchannel and an additional anti-fouling step in the protocol. DNA sequence detection is achieved through a hybridization sandwich of an immobilized complementary probe, the target DNA sequence, and a second complementary probe labeled with beta-galactosidase (beta-GAL); the beta-GAL converts its substrate, 4-aminophenyl-D-galactopyranoside (PAPG), into PAP. In this report we present the lowest reported observed detection limit (1.0 x 10(-10) M) for an enzyme-linked DNA hybridization assay using an IDA microelectrode and a redox signaling paradigm. Thus, we have demonstrated highly sensitive detection of a targeted DNA sequence using a low-cost easily fabricated electrochemical biosensor integrated into a microfluidic channel. (C) 2011 Elsevier B.V. All rights reserved.
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