期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 6, 页码 2870-2876出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.11.030
关键词
Locked nucleic acids; Electrochemical biosensor; Sandwich sensing mode; Acute promyelocytic leukemia; Enzyme; Mixed hybridization system
类别
资金
- National High Technology and Development of China [2008AA02Z433]
- National Natural Science Foundation of China [20805006, 20975021]
- Fujian Provincial University - Industry Cooperation Science Technology [2010Y4003]
- Fujian Provincial Important Science and Technology Foundation [2008Y0045]
- Fujian Medical University [09ZD013]
- Natural Science Foundation of Fujian Province of China [2010J06011]
- Fujian Province University [JA10126]
- Nursery Foundation of Fujian Medical University [2010MP043]
In this study, a novel DNA electrochemical probe (locked nucleic acid, LNA) was designed and involved in constructing an electrochemical DNA biosensor for detection of promyelocytic leukemia/retinoic acid receptor alpha (PML/RAR alpha) fusion gene in acute promyelocytic leukemia for the first time. This biosensor was based on a 'sandwich' sensing mode, which involved a pair of LNA probes (capture probe immobilized at electrode surface and biotinyl reporter probe as an affinity tag for streptavidin-horseradish peroxidase (streptavidin-HRP). Since biotin can be connected with streptavidin-HRP, this biosensor offered an enzymatically amplified electrochemical current signal for the detection of target DNA. In the simple hybridization system, DNA fragment with its complementary DNA fragment was evidenced by amperometric detection, with a detection limit of 74 fM and a linear response range of 0.1-10 pM for synthetic PML/RAR alpha fusion gene in acute promyelocytic leukemia (APL). Otherwise, the biosensor showed an excellent specificity to distinguish the complementary sequence and different mismatch sequences. The new pattern also exhibited high sensitivity and selectivity in mixed hybridization system. (C) 2010 Elsevier B.V. All rights reserved.
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