期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 5, 页码 2666-2669出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.03.013
关键词
Mercury ions; Label-free detection; Polymerase; Fluorescence amplification
类别
资金
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0829]
- NSFC-Guangdong [U0931005]
- National Basic Research Program of China [2010CB732602]
- National High Technology Research and Development Program of China (863 Program) [2007AA10Z204]
A new label-free Hg2+ ions assay with polymerase assisted fluorescence amplification was developed. In this approach, a single strand template probe containing a mercury ion recognition sequence (MRS) which is composed of two thymine (T)-rich functional areas separated by a spacer of random bases was designed. In this sensing system, upon addition of Hg2+ ions, the complexation of Hg2+ ions with the MRS yielded a hairpin complex through T-Hg2+-T base pairs. The formation of the hairpin structure initiated the replication of the template probe in the presence of the polymerase/dNTPs. Then the replication products of long duplex DNA probes were stained with a sensitive reagent SYBR Green I (SG). As a result, SG integrated with the duplex DNA probes and induced a distinguishable fluorescence enhancement in response to Hg2+ ions triggered replication reaction. Due to the fluorescence amplification step, this method exerted substantial enhancement in sensitivity and could be used for rapid and selective detection of low picomolar Hg2+ ions. It is expected that this cost-effective fluorescence sensor might hold considerable potential in the detection of Hg2+ ions in real environmental samples. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.
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