期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 5, 页码 2090-2096出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.09.012
关键词
Brevetoxins; Electrochemical immunosensor; Food safety; Gold nanoparticles; Poly(amidoamine) dendrimer
类别
资金
- National Basic Research Program of China [2010CB732403]
- National Natural Science Foundation of China [21075019, 20877019]
- Key Natural Sciences Foundation of Fujian Province, China [D0520001]
- Science and Technology Department of Fujian Province, China [2007Y0026]
- NTU-MOE [RG65/08]
- Fuzhou University [0460-022275]
A sensitive electrochemical immunosensor for the fast screening of brevetoxin B (BTX-2) in food samples was developed by means of immobilizing BTX-2-bovine serum albumin conjugate (BTX-2-BSA) on the gold nanoparticles-decorated amine-terminated poly(amidoamine) dendrimers (AuNP-PAADs). The presence of gold nanoparticles greatly improved the conductivity of the PAADs, and three-dimensional PAADs increased the surface coverage of the biomolecules on the electrode. Under optimal conditions, three types of immunosensor, i.e. with AuNPs, PAADs, or AuNP-PAADs, were used for the determination of BTX-2 in a competitive-type immunoassay format using horseradish peroxidase-labeled anti-BTX antibodies (HRP-anri-BTX-2) as trace in the H(2)O(2)-o-phenylenediamine (o-PD) system. A low detection limit (LOD) of 0.01 ng/mL and a wide dynamic working linear range of 0.03-8 ng/mL BTX-2 using AuNP-PAADs as matrices were obtained in comparison with those of only using AuNP or PAADs. Intra-batch assay precision was substantially improved by resorting to the AuNP-PAADs manifold. The proposed method features unbiased identification of negative (blank) and positive samples. No significant differences were encountered in the analysis of the spiking real samples between the electrochemical immunosensor and liquid chromatography for the determination of BTX-2. Importantly, this method provided a biocompatible immobilization and a promising immunosensing platform for analytes with small molecules in the analysis and detection of food safety. (C) 2010 Elsevier B.V. All rights reserved.
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